Iron Diagnostic Kit is intended for the quantitative determination of iron concentration in human serum or plasma using photometric methods on clinical chemistry analyzers, photometers, and spectrophotometers. The assay is based on the Ferrozine method, in which iron is released from transferrin in an acidic medium, reduced to ferrous ions by ascorbic acid, and subsequently forms a colored complex with Ferrozine. The intensity of the color measured at 570 nm is directly proportional to iron concentration in the sample.
Method
Ferrozine Colorimetric Method
Sample Type
Serum / Plasma (Heparinized plasma only)
EDTA, oxalate, and citrate plasma are not suitable
Storage
2–8°C
(Protect from light. Do not freeze.)
Catalog Numbers
010287004300 (H917)
010287004500 (MPR)